Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats

Abstract

Abstract Objective The present study was performed to evaluate the protective effects of methanol extract of Cedrus deodara against cisplatin induced. Methods Myelosuppression in albino Wistar rats. All experimental animals were administered with cisplatin on 1st, 3rd, 5th and 7th day to induce bone marrow toxicity and rats were treated with methanol extract of C. deodara for 21 days. Blood samples were collected from all the animals on day 1st, 7th, 14th and 21st after 1 h before the administration of the drugs and hematological parameters like RBC, WBC, platelets, hemoglobin, hematocrit, eosinophils, basophils, neutrophils, lymphocytes, bleeding time and clotting time were determined were determined. At 21st, all rats were sacrificed and bone marrow samples were collected. The part of bone marrow samples was used for the determination of antioxidant enzymes and remaining were subjected to histopathological examination. Results The animals of therapeutic groups administered with extract of C. deodara have exhibited significant rise in hematological parameters and shorten bleeding time and clotting time when compare to toxic control animals on the day 14 and day 21. The histopathological examination revealed the regeneration of bone marrow cells in the extract treated animals. There was significant reduction in lipid peroxidation and increase in antioxidant enzymes was found in extract treated animals. Conclusions The methanol extract of C. deodara of have shown significant protective effects against cisplatin induced myelosuppression in albino Wistar rats.