Unimpaired de novo Synthesis of 4-Aminobenzoate in a Mutant of Aerobacter aerogenes Requiring 4-Aminobenzoate for Growth; Structure of Compound A

Author:

Bacher A.,Gilch B.,Rappold H.,Lingens F.

Abstract

Abstract The mutant strain Aerobacter aerogenes 62-1 AC, pab-, accumulates a labile substance, desig­nated Compound A which supports the growth of other pab- mutants. Previous studies seemed to indicate that Compound A might be an intermediate in the conversion of chorismate to 4-amino-benzoate (Altendorf, Bacher, and Lingens, Z. Naturforsch. 24 b, 1602 [1969]). The present experi­ ments show that Compound A is 4 -(D-glucosylamino)-benzoic acid. This substance which is clearly not an intermediate in the biosynthesis of 4-aminobenzoate is formed by reaction of 4-aminobenzoate produced de novo by the mutant with exogenous glucose. Mutant 62-1 AC has the unimpaired capacity to synthesize 4-aminobenzoate as shown by direct enzyme studies. Mutant 62-1 AC re­ quires approximately 200 times more 4jaminobenzoate for growth than other pab" mutants of A. aerogenes with defective 4-aminobenzoate synthetase. The genetic defect of mutant 62-1 AC seems to be located in a metabolic system concerned with the utilization of 4-aminobenzoate rather than its synthesis.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

Cited by 5 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The Folate Precursor p-Aminobenzoate Is Reversibly Converted to Its Glucose Ester in the Plant Cytosol;Journal of Biological Chemistry;2003-06

2. para-Aminobenzoate Synthesis from Chorismate Occurs in Two Steps;Journal of Biological Chemistry;1989-05

3. Dislocations in energetic materials Dislocation characterization and post-growth motion in single crystals of cyclotrimethylene trinitramine;Philosophical Magazine A;1986-04

4. X-ray Diffraction Topography of Growth Defects in Crystals;Рост Кристаллоь / Rost Kristallov / Growth of Crystals;1984

5. Defects in Non-Metal Crystals;Characterization of Crystal Growth Defects by X-Ray Methods;1980

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3