Imaging of photoassimilates transport in plant tissues by positron emission tomography

Author:

Partelová Denisa,Kuglerová Klára,Konotop Yevheniia,Horník Miroslav,Lesný Juraj,Gubišová Marcela,Gubiš Jozef,Kováč Peter,Matušíková Ildikó

Abstract

AbstractThe current findings show that positron emission tomography (PET), primarily developed for medical diagnostic imaging, can be applied in plant studies to analyze the transport and allocation of wide range of compounds labelled with positronemitting radioisotopes. This work is focused on PET analysis of the uptake and transport of 2-deoxy-2-fluoro[18F]-D-glucose (2-[18F]FDG), as a model of photoassimilates, in tissues of giant reed (Arundo donax L. var. versicolor) as a potential energy crop. The absorption of 2-[18F]FDG and its subsequent transport in plant tissues were evaluated in both acropetal and basipetal direction as well. Visualization and quantification of the uptake and transport of 2-[18F]FDG in plants immersed with the root system into a 2-[18F]FDG solution revealed a significant accumulation of 18F radioactivity in the roots. The transport rate in plants was increased in the order of plant exposure through: stem > mechanically damaged root system > intact root system. PET analysis in basipetal direction, when the plant was immersed into the 2-[18F]FDG solution with the cut area of the leaf of whole plant, showed minimal translocation of 2-[18F]FDG into the other plant parts. The PET results were verified by measuring the accumulated radioactivity of18F by direct gamma-spectrometry.

Publisher

Walter de Gruyter GmbH

Subject

Health, Toxicology and Mutagenesis,Food Science,Biotechnology,Analytical Chemistry

Reference19 articles.

1. deoxy fluoro - glucose metabolism in Arabidopsis thaliana;Fatangare;Front Plant Sci,2015

2. In situ imaging of metals in cells and tissues;McRae;Chem Rev,2009

3. Imaging of radiocesium uptake dynamics in a plant body by using a newly developed high - resolution gamma camera;Kawachi;Environ Radioact,2016

4. Giant reed donax weed plant or a promising energy crop;Pilu;Afr J Biotechnol,2012

5. Incorporation and translocation of deoxy fluoro - glucose in Sorghum bicolour Moench monitored using a planar positron imaging system;Hattori;Planta,2008

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3