Affiliation:
1. Department of Population and Evolutionary Biology, University of Utrecht, Padualaan 8, Utrecht, The Netherlands
Abstract
Abstract
In green parts of Silene plants of the genotype glR two methylated flavonoids were found: isoscoparin and isosco-parin 2″-O-rhamnoside. An enzyme has been demonstrated to catalyze the transfer of the methyl moiety of S-adenosyl methionine to iso-orientin and iso-orientin 2″-O-rhamnosi-de. Maximal activity takes place at pH 8.0 -8 .2 . Of the metal ions Mn2+, Mg2+, Ca2+, Co2+, Zn2+ and Hg2+, only Co2+ stimulated the reaction at conc. > 2 mM . For the methyla tion of isoorientin the Km values were 4 × 10-6 M for S-ade-nosyl methionine and 0.32 × 10-3 m for iso-orientin. When isoorientin 2″-O-rhamnoside was used as substrate the Km values were 5 × 10-6 m for S-adenosylmethionine and 7 × 10-6 M for iso-orientin 2″-O-rhamnoside.
Subject
General Biochemistry, Genetics and Molecular Biology
Cited by
13 articles.
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