Contribution of Mevalonate and Methylerythritol Phosphate Pathways to Polyisoprenoid Biosynthesis in the Rubber-Producing Plant Eucommia ulmoides Oliver

Author:

Bamba Takeshi12,Murayoshi Michiko32,Gyoksen Koichirou3,Nakazawa Yoshihisa2,Okumoto Hiroshi4,Katto Hiroko4,Fukusaki Eiichiro1,Kobayashi Akio1

Affiliation:

1. Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 - 1 Yamadaoka, Suita, Osaka 565-0871, Japan

2. Hitachi Zosen Corporation, 2-2-11 Funamachi, Taisyou-ku, Osaka 551-0022, Japan

3. Department of Forest and Forest Products Sciences, Faculty of Agriculture, Graduate Schools of Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan

4. Kurashiki University of Science and The Arts, Department of Chemistry and Bioscience, 2640 Nishinoura Tsurajima, Kurashiki, Okayama 712-8505, Japan

Abstract

The biosynthetic origin of isopentenyl diphosphate in the polyisoprenoid biosynthesis of the rubber-producing plant Eucommia ulmoides Oliver was elucidated for the fi rst time by feeding experiments using 13C-labeled isotopomers of (RS)-mevalonate, 1-deoxy-D-xylulose- 3,4,5-triacetate, 2C-methyl-D-erythritol-1,2,3,4-tetraacetate, and pyruvate. After 13C-labeled isotopomers were fed to the young seedlings, the polyisoprenoid fractions were prepared and analyzed by 13C NMR. The NMR data showed that the isoprene units of polyisoprenoid derived from isopentenyl diphosphate, which was biosynthesized using both mevalonate and 1-deoxy-D-xylulose-5-phosphate in E. ulmoides. It is assumed that the cross-talk of isopentenyl diphosphate, derived from both pathways, occurs during the biosynthesis of polyisoprenoid; therefore, it was observed in the formation of low-molecular weight isoprenoids.

Publisher

Walter de Gruyter GmbH

Subject

General Biochemistry, Genetics and Molecular Biology

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