Mass spectrometry of membrane protein complexes
Author:
Bender Julian1ORCID, Schmidt Carla1ORCID
Affiliation:
1. Interdisciplinary Research Center HALOmem, Charles Tanford Protein Centre , Martin Luther University Halle-Wittenberg, Institute for Biochemistry and Biotechnology , Kurt-Mothes-Str. 3a , D-06120 Halle , Germany
Abstract
Abstract
Membrane proteins are key players in the cell. Due to their hydrophobic nature they require solubilising agents such as detergents or membrane mimetics during purification and, consequently, are challenging targets in structural biology. In addition, their natural lipid environment is crucial for their structure and function further hampering their analysis. Alternative approaches are therefore required when the analysis by conventional techniques proves difficult. In this review, we highlight the broad application of mass spectrometry (MS) for the characterisation of membrane proteins and their interactions with lipids. We show that MS unambiguously identifies the protein and lipid components of membrane protein complexes, unravels their three-dimensional arrangements and further provides clues of protein-lipid interactions.
Publisher
Walter de Gruyter GmbH
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
Reference132 articles.
1. Akashi, S., Shirouzu, M., Terada, T., Ito, Y., Yokoyama, S., and Takio,K. (1997). Characterization of the structural difference between active and inactive forms of the Ras protein by chemical modification followed by mass spectrometric peptide mapping. Anal. Biochem. 248, 15–25. 2. Ambrose, S., Housden, N.G., Gupta, K., Fan, J., White, P., Yen, H., Marcoux, J., Kleanthous, C., Hopper, J.T.S., and Robinson, C.V. (2017). Native desorption electrospray ionization liberates soluble and membrane protein complexes from surfaces. Angew. Chem. Int. Ed. 129, 14655–14660. 3. Arlt, C., Flegler, V., Ihling, C.H., Schafer, M., Thondorf, I., and Sinz,A. (2017). An integrated mass spectrometry based approach to probe the structure of the full-length wild-type tetrameric p53 tumor suppressor. Angew. Chem. Int. Ed. 56, 275–279. 4. Barrera, N.P., Di Bartolo, N., Booth, P.J., and Robinson, C.V. (2008). Micelles protect membrane complexes from solution to vacuum. Science 321, 243–246. 5. Barrera, N.P., Isaacson, S.C., Zhou, M., Bavro, V.N., Welch, A., Schaedler, T.A., Seeger, M.A., Miguel, R.N., Korkhov, V.M., van Veen, H.W., et al. (2009). Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions. Nat. Methods 6, 585–587.
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