Conversion of chenodeoxycholic acid to cholic acid by human CYP8B1-Reference-Cited by-同舟云学术

Conversion of chenodeoxycholic acid to cholic acid by human CYP8B1

Published:2018-12-18 Issue:5 Volume:400 Page:625-628
ISSN:1437-4315
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Fan Linbing¹,Joseph Jan Felix²³,Durairaj Pradeepraj¹,Parr Maria Kristina²,Bureik Matthias¹

Affiliation:

1. School of Pharmaceutical Science and Technology, Health Sciences Platform , Tianjin University , Tianjin 30072 , China

2. Freie Universitaet Berlin, Institute of Pharmacy , Pharmaceutical and Medicinal Chemistry (Pharmaceutical Analyses) , Berlin , Germany

3. Freie Universitaet Berlin , Department of Biology, Chemistry, Pharmacy, Core Facility BioSupraMol , Berlin , Germany

Abstract

Abstract The human cytochrome P450 enzyme CYP8B1 is a crucial regulator of the balance of cholic acid (CA) and chenodeoxycholic acid (CDCA) in the liver. It was previously shown to catalyze the conversion of 7α-hydroxycholest-4-en-3-one, a CDCA precursor, to 7α,12α-dihydroxycholest-4-en-3-one, which is an intermediate of CA biosynthesis. In this study we demonstrate that CYP8B1 can also convert CDCA itself to CA. We also show that five derivatives of luciferin are metabolized by CYP8B1 and established a rapid and convenient inhibitor test system. In this way we were able to identify four new CYP8B1 inhibitors, which are aminobenzotriazole, exemestane, ketoconazole and letrozole.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Reference16 articles.

1. Bhatnagar, A.S., Hausler, A., Schieweck, K., Lang, M., and Bowman, R. (1990). Highly selective inhibition of estrogen biosynthesis by CGS 20267, a new non-steroidal aromatase inhibitor. J. Steroid Biochem. Mol. Biol. 37, 1021–1027.

2. Cali, J.J., Ma, D., Wood, M.G., Meisenheimer, P.L., Klaubert, D.H. (2012). Bioluminescent assays for ADME evaluation: dialing in CYP selectivity with luminogenic substrates. Expert Opin. Drug Metab. Toxicol. 8, 1115–1130.

3. Chevre, R., Trigueros-Motos, L., Castano, D., Chua, T., Corliano, M., Patankar, J.V., Sng, L., Sim, L., Juin, T.L., Carissimo, G., et al. (2018). Therapeutic modulation of the bile acid pool by Cyp8b1 knockdown protects against nonalcoholic fatty liver disease in mice. FASEB J. 32, 3792–3802.

4. Diani-Moore, S., Papachristou, F., Labitzke, E., and Rifkind, A.B. (2006). Induction of CYP1A and cyp2-mediated arachidonic acid epoxygenation and suppression of 20-hydroxyeicosatetraenoic acid by imidazole derivatives including the aromatase inhibitor vorozole. Drug Metab. Dispos. 34, 1376–1385.

5. Dragan, C.-A., Zearo, S., Hannemann, F., Bernhardt, R., and Bureik, M. (2005). Efficient conversion of 11-deoxycortisol to cortisol (hydrocortisone) by recombinant fission yeast Schizosaccharomyces pombe. FEMS Yeast Res. 5, 621–625.

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