Author:
Wałecka-Zacharska E.,Kosek-Paszkowska K.,Bania J.,Staroniewicz Z.,Bednarski M.,Wieliczko A.
Abstract
AbstractAnimals are important reservoir ofListeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness ofL. monocytogenesstrains of animal origin is very scarce. Ability of 18L. monocytogenesstrains of animal origin to invade HT-29 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% ofL. monocytogenesstrains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1/2b consisted of strains of invasiveness below 0.0001%. Analysis of translatedinlAandinlBgene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified withininlAandinlBsequences. Our results demonstrate high incidence of low invasive animalL. monocytogenesstrains, which may be at least partly explained by unique point mutations in the InlA and InlB.
Subject
General Veterinary,General Medicine
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