Frequency of iss and irp2 genes by PCR method in Escherichia coli isolated from poultry with colibacillosis in comparison with healthy chicken in poultry farms of Zabol, South East of Iran

Abstract

AbstractThere is no special trait for differentiation of Avian PathogenicEscherichia colifrom Avian FecalEscherichia coli. This investigation is aimed, as a case control study, to evaluate and compare the frequency ofissandirp2in 43 AFEC strains and also 40 and 56E. colistrains isolated from the liver and kidney of chickens with colibacillosis, respectively, farmed in Zabol, as a border region of Iran, by PCR. 86.9% and 37.2% of isolates collected from chickens with colibacillosis and feces samples obtained from healthy chickens were positive forissgene, respectively (P<0.05). On average, 59.3% ofE. colistrains isolated from colibacillosis haveirp2gene while 27.9% of isolates from the feces of healthy birds were positive (P<0.05). 52.15% of isolates from colibacillosis and 19.62% of isolates from healthy chicken feces were positive for both genes, with statistical significant difference (p<0.05). This marked difference in the distribution ofissandirp2genes makes these two genes good markers to differentiate AFEC and APEC strains especially in Sistan region to improve colibacillosis control measurements.