Structure and phase separation of the C-terminal domain of RNA polymerase II
Author:
Lushpinskaia Irina P.1, Flores-Solis David1, Zweckstetter Markus12ORCID
Affiliation:
1. German Center for Neurodegenerative Diseases (DZNE) , Von-Siebold Straße 3A, D-35075 Göttingen , Germany 2. Department of NMR-based Structural Biology , Max Planck Institute for Multidisciplinary Sciences , Am Faßberg 11, D-37077 Göttingen , Germany
Abstract
Abstract
The repetitive heptads in the C-terminal domain (CTD) of RPB1, the largest subunit of RNA Polymerase II (Pol II), play a critical role in the regulation of Pol II-based transcription. Recent findings on the structure of the CTD in the pre-initiation complex determined by cryo-EM and the novel phase separation properties of key transcription components offers an expanded mechanistic interpretation of the spatiotemporal distribution of Pol II during transcription. Current experimental evidence further suggests an exquisite balance between CTD’s local structure and an array of multivalent interactions that drive phase separation of Pol II and thus shape its transcriptional activity.
Funder
Deutsche Forschungsgemeinschaft
Publisher
Walter de Gruyter GmbH
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
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