Crosstalk between cholesterol and PIP<sub>2</sub> in the regulation of Kv7.2/Kv7.3 channels-Reference-Cited by-同舟云学术

Crosstalk between cholesterol and PIP₂ in the regulation of Kv7.2/Kv7.3 channels

Published:2023-08-08 Issue:0 Volume:0 Page:
ISSN:1431-6730
Container-title:Biological Chemistry
language:en
Short-container-title:

Author:

Delgado-Ramírez Mayra¹,López-Serrano Ana Laura¹,Sánchez-Armass Sergio¹,Meza Ulises¹,Rodríguez-Menchaca Aldo A.¹^ORCID

Affiliation:

1. Departamento de Fisiología y Biofísica, Facultad de Medicina , Universidad Autónoma de San Luis Potosí , Av. Venustiano Carranza #2405, Col. Los Filtros , San Luis Potosí , SLP , 78210 , México

Abstract

Abstract The activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that currents passing through Kv7.2/Kv7.3 channels in cholesterol-depleted cells, by acute application of methyl-β-cyclodextrin (MβCD), were less sensitive to PIP2 dephosphorylation strategies than those of control cells, suggesting that cholesterol depletion enhances the Kv7.2/Kv7.3–PIP2 interaction. In contrast, the sensitivity of Kv7.2/Kv7.3 channels to acute membrane cholesterol depletion by MβCD was not altered in mutant channels with different apparent affinities for PIP2.

Funder

SEP-CONACYT

Universidad Autónoma de San Luis Potosí

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/hsz-2023-0204/pdf

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