Phenol Oxidase Activity and One-Electron Oxidation Activity in Wood Degradation by Soft-Rot Deuteromycetes

Abstract

Summary Wood degradation, one-electron oxidation activity as assayed by ethylene production from 2-keto-4-thiomethylbutyric acid, and phenol oxidase activity were measured in cultures of six deuteromycete fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing cultures. These four fungi also had measurable phenol oxidase activity in wood-containing cultures, but not in glucose-containing cultures. The two mould fungi that did not significantly degrade wood had no phenol oxidase activity in either wood- or glucose-containing cultures. The one-electron oxidation activity in intact cultures of the soft-rot deuteromycetes was roughly related with the rate of weight loss during wood degradation in those cultures. However, there was no clear relationship between phenol oxidase activity and either one-electron oxidation activity or the rate of wood weight loss, either over time, or in total. Most of the one-electron oxidation activity resulted from phenol oxidase and hydroxyl radical. Most of the phenol oxidase activity resulted from laccase.