Affiliation:
1. College of Science, Department of Chemistry , University of Hafr Al Batin , P.O. Box 1803, 39524 Al Jamiah District , Hafr Al Batin , Kingdom of Saudi Arabia
Abstract
Abstract
This study describes the in-situ growth of cobalt nanoparticles (Co NPs) to a naked-eye determination of tetracycline antibiotics in the water, honey, and milk samples as a colorimetric method. Tetracycline antibiotics bonded cobalt NPs (CoNPs-TCs) are performed light green to yellow color with high UV–vis absorbance at lambda maximum of 400 nm. Cobalt nanoparticles bonded tetracycline were characterized by using UV–vis spectroscopy, Fourier transforms infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), and field-emission scanning electron microscopy (FE-SEM). Effective parameters of the method, such as solution pH (∼8.5), reaction time (3 min), temperature (25 °C), and cobalt concentration (20 μg mL−1) were studied. Analytical validation is performed that the limit of determination 0.21–1.09 μg mL−1 and the limit of quantification was 0.73–3.88 μg mL−1 with a matrix match linear dynamic range from 1 to 100 μg mL−1 for tetracyclines in all water, milk, and honey samples. The accuracy and precision of the method were evaluated with real samples recovery (80–108 %, ±5) and precision (intraday, 0.73–3.75 %, n = 3 and interday, 2.01–4.82 %, n = 9), respectively. This method provides a rapid, simple, and inexpensive detection with acceptable sensitivity.
Subject
Engineering (miscellaneous),Food Science,Biotechnology
Reference29 articles.
1. Aljedani, DM. Antibiotic treatment (Tetracycline) effect on bio-efficiency of the larvae honey bee (Apis mellifera jemenatica). Saudi J Biol Sci 2022;29:1477–86. https://doi.org/10.1016/j.sjbs.2021.11.024.
2. Dabagh Moghadam, A, Taiiebi, L, Falahat Pishe, HR, Mahmudian, M, Kousari, N. The determination of tetracycline residues in pasteurized milk distributed in tehran using high performance liquid chromatography. AJA Univ Med Sci 2013;4:318–23.
3. Palczynski, LJ, Bleach, ECL, Brennan, ML, Robinson, PA. Stakeholder perceptions of disease management for dairy calves: “it’s just little things that make such a big difference”. Animals 2021;11:2829. https://doi.org/10.3390/ani11102829.
4. Baxi, VK, Gadani, DH, Rana, VA. Dielectric properties of honey adulterated by sugar syrup. Int J Food Eng 2022;18:603–9. https://doi.org/10.1515/ijfe-2022-0063.
5. Chopra, I, Roberts, M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev 2001;65:232–60. https://doi.org/10.1128/mmbr.65.2.232-260.2001.