Beyond Dinitrophenol Interaction with Tryptophan-Based Compounds-Reference-Cited by-同舟云学术

Beyond Dinitrophenol Interaction with Tryptophan-Based Compounds

Published:2016-07-01 Issue:1 Volume:24 Page:43-49
ISSN:2067-2446
Container-title:Acta Chemica Iasi
language:en
Short-container-title:

Author:

Zaharia Marius¹,Drochioiu Gabi¹,Zbancioc Gheorghiță¹,Grădinaru Vasile Robert¹

Affiliation:

1. Department of Chemistry, “Al. I. Cuza” University Iasi, 11 Carol I Bd Iasi 700506, Romania

Abstract

Abstract The effects of 2,4-dinitrophenol (2,4-DNP) on the spectroscopic parameters (UV-Vis or FT-IR absorbance) or fluorescence emission of tryptophan and glycyl-tryptophan were studied. A quenching phenomenon of fluorescence was observed, attributed to interactions between the indole ring of the fluorophore and the aromatic ring of the quencher. The analysis of fluorescence spectra confirms that the quenching is dictated by 2,4-DNP concentration and pH. A combined mechanism of static and dynamic quenching was detected. The quenching phenomenon observed in this work could be employed to explain the mechanism of action of such compounds on large fluorescent peptides or proteins.

Publisher

Walter de Gruyter GmbH

Reference9 articles.

1. 1. Badeen, C.; Turcotte, R.; Hobenshield, E.; Berretta, S. Thermal hazard assessment of nitrobenzene/dinitrobenzene mixtures. J. Hazard. Mater. 2011, 188, 52-57.

2. 2. Yen, M.; Ewald, M.B. Toxicity of weight loss agents. J. Med. Toxicol. 2012, 8(2), 145-152.

3. 3. Grundlingh, J., Dargan, P. I., El-Zanaly, M., and Wood, D. 2,4-Dinitrophenol (DNP): A weight loss agent with significant acute toxicity and risk of death. J. Med. Toxicol. 2011, 3, 205-212.

4. 4. Ennis, S.R.; Keep, R.F. Effects of 2,4-dinitrophenol on ischemia-induced blood-brain barrier disruption. Acta Neurochir. Suppl. 2006, 96, 295-298.

5. 5. Vier, J.; Gerhard, M.; Wagner, H., Häcker, G. Enhancement of death-receptor induced caspase-8-activation in the death-inducing signalling complex by uncoupling of oxidative phosphorylation. Mol. Immunol. 2004, 40(10), 661-670.