Collection, transport and storage procedures for blood culture specimens in adult patients: recommendations from a board of Italian experts

Author:

De Plato Francesca1,Fontana Carla23,Gherardi Giovanni4,Privitera Gaetano Pierpaolo56,Puro Vincenzo7,Rigoli Roberto8,Viaggi Bruno9,Viale Pierluigi10

Affiliation:

1. Società Italiana Farmacologia Ospedaliera , Milan , Italy ; and Local Health Authority ASL of Teramo , Teramo , Italy

2. Department of Experimental Medicine , University of Rome Tor Vergata , Rome , Italy

3. Microbiology and Virology Laboratory , Polyclinic of Tor Vergata , Rome , Italy

4. Department of Medicine, Laboratory of Clinical Microbiology , University Campus Biomedico , Rome 00128 , Italy

5. Department of Translational Research and New Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy

6. University Hygiene and Epidemiology Complex Operative Unit and Clinical Risk Functional Area Coordinator, University Hospital Pisana , Pisa , Italy

7. National Institute for Infectious Diseases L. Spallanzani, IRCCS , Rome , Italy

8. Department of Clinical Pathology , Local Health and Social Care Facility, No. 2, Marca Trevigiana , Treviso , Italy

9. Neuroanesthesia and Intensive Care Unit , Careggi University Hospital , Florence , Italy

10. Department of Medical and Surgical Sciences , Alma Mater Studiorum University of Bologna , Bologna , Italy

Abstract

Abstract Bloodstream infections (BSIs) remain a potentially life-threatening condition. The gold standard for the diagnosis of BSI is still blood cultures (BCs), and the diagnostic yield depends on clinical and technical factors that have an impact on collection and transportation. Hence, monitoring of the entire pre-analytical process from blood collection to transportation to the microbiology laboratory is critical. To optimize the clinical impact of the diagnostic and therapeutic procedures, a multidisciplinary approach and univocal protocols are mandatory. A board of specialists discussed the available evidence on the pre-analytical process and produced the present document to guide physicians and nurses on the ideal execution of BC: (1) timing and preparation for blood collection; (2) skin antisepsis; (3) blood volume; (4) sampling method and safety; (5) medium to be used; (6) time to BC transportation; and (7) quality assurance and quality management.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,General Medicine

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