Rapid detection of non-deletional mutations causing α-thalassemia by multicolor melting curve analysis

Author:

Huang Qiuying,Wang Xudong,Tang Ning,Zhu Chunjiang,Yan Tizhen,Chen Ping,Li Qingge

Abstract

AbstractThe assay consisted of one pair of primers specific for theThe assay had a reproducibility of 100%, could detect gDNA of different genotype as low as 1 ng per reaction, and had an overall accuracy of 100% when compared with RDB analysis and Sanger sequencing.The developed assay is rapid, robust, and cost-effective while maintaining high sensitivity, specificity, and throughput.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,General Medicine

Reference32 articles.

1. disease induced by the common SEA deletion and the rare hemoglobin Quong Sze in a Thai female : longitudinal clinical course molecular characterization and development of a PCR RFLP - based detection method;Sura;Hemoglobin Ann Hematol,2007

2. SS Multiple minisequencing screen for seven southeast Asian nondeletional alpha - thalassemia mutations;Wang;Clin Chem,2003

3. DR Alpha thalassaemia;Harteveld;Orphanet J Rare Dis,2010

4. Multiplex fluorescence melting curve analysis for mutation detection with dual - labeled self - quenched probes One;Huang,2011

5. reverse dot - blot method for rapid detection of non - deletion alpha thalassaemia;Chan,1999

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