Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors-Reference-Cited by-同舟云学术

Expression levels of BAP1, OGT, and YY1 genes in patients with eyelid tumors

Published:2021-12-01 Issue:6 Volume:46 Page:709-719
ISSN:1303-829X
Container-title:Turkish Journal of Biochemistry
language:en
Short-container-title:

Author:

Tas Ayca¹^ORCID,Gumus Erkan²^ORCID,Ozmen Esma³^ORCID,Erdogan Haydar⁴^ORCID,Silig Yavuz⁵^ORCID

Affiliation:

1. Department of Nutrition and Diet , Faculty of Health Sciences, Sivas Cumhuriyet University , Sivas , Turkey

2. Department of Histology and Embryology , Faculty of Medicine, Adnan Menderes University , Aydın , Turkey

3. Department of Medical Biochemistry , Faculty of Medicine, Nigde Omer Halis Demir University , Nigde , Turkey

4. Department of Ophthalmology , Faculty of Medicine, Sivas Cumhuriyet University , Sivas , Turkey

5. Department of Medical Biochemistry , Faculty of Medicine, Sivas Cumhuriyet University , Sivas , Turkey

Abstract

Abstract Objectives The aim of this study was to investigate BAP1, OGT and YY1 genes and protein levels in 12 samples (8 males, 4 females) of eyelid tumor tissue with basal cell carcinoma (BCC) and 12 normal control subjects (8 males, 4 females). Methods The expression levels of these genes were determined with RT-PCR and the protein levels and expression using ELISA and IHC methods, respectively. Results In RT-PCR analysis, statistically significant upregulated expression was determined of 1.84-fold of BAP1, 2.85-fold of OGT and 3.06-fold of YY1 genes (p < 0.05). In the patient group, compared to the control group, there was a similar statistically significant strong correlation between the proteins (BAP1 and YY1; r = 0.850, BAP1 and OGT; r = 0.811, OGT and YY1; r = 0.755) (p < 0.05). In the ELISA and IHC analysis methods, a significant increase in BAP1 and YY1 protein expression levels was observed compared to the control group (p < 0.05). Conclusions The study results demonstrated that BAP1 and YY1 genes and protein levels were upregulated in eyelid tumor tissue with BCC.

Publisher

Walter de Gruyter GmbH

Subject

Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/tjb-2021-0160/pdf

Reference66 articles.

1. Cook, BEJr, Bartley, GB. Epidemiologic characteristics and clinical course of patients with malignant eyelid tumors in an incidence cohort in Olmsted County, Minnesota. Ophthalmology 1999;106:746–50. https://doi.org/10.1016/s0161-6420(99)90161-6.

2. Lin, HY, Cheng, CY, Hsu, WM, Kao, WM, Chou, P. Incidence of eyelid cancers in Taiwan: a 21-year review. Ophthalmology 2006;113:2101–7. https://doi.org/10.1016/j.ophtha.2006.06.001.

3. Damasceno, JC, Isenberg, J, Lopes, LR, Hime, B, Fernandes, BF, Lowen, M, et al.. Largest case series of Latin American eyelid tumors over 13-years from a single center in Sao Paulo, Brazil. Arq Bras Oftalmol 2018;81:7–11. https://doi.org/10.5935/0004-2749.20180004.

4. Chinem, VP, Miot, HA. Epidemiology of basal cell carcinoma. An Bras Dermatol 2011;86:292–305. https://doi.org/10.1590/s0365-05962011000200013.

5. Helgadottir, H, Höiom, V. The genetics of uveal melanoma: current insights. Appl Clin Genet 2016;9:147. https://doi.org/10.2147/TACG.S69210.