Affiliation:
1. Amasya University Faculty of Medicine , Department of Medical Biochemistry , Amasya , Turkey
2. The University of Health Sciences Ankara Diskapi Yildirim Beyazit Training and Research Hospital , Clinical Biochemistry Laboratory , Ankara , Turkey
3. Omer Halisdemir University Training and Research Hospital , Clinical Biochemistry Laboratory , Nigde , Turkey
Abstract
Abstract
Objectives
This study aimed to verify the enzymatic ethanol analysis method and compare the technique with headspace gas chromatography (HS/GC).
Methods
The limit of the blank, limit of detection, and limit of quantification, precision, linearity, and accuracy of the enzymatic process were evaluated based on the data obtained. In addition, a method comparison was made between the enzymatic method and the HS/GC analysis method.
Results
It was seen that the enzymatic method was linear in the range of 14–450 mg/dL, the coefficient of variation and bias values ranged between 0.5–1.09% and 4.52–9.78%, respectively. The quantitative accuracy was 2.13% and 2.23% at concentrations of 41.16 and 95.82 mg/dL, respectively. When the regression analysis was performed between the results obtained using the enzymatic alcohol dehydrogenase (ADH) and HS/GC methods, y=0.966x + 2.084 linear relationship formula and regression coefficient of r2=0.9987, it was found that it showed a good agreement at a confidence interval of 5%.
Conclusions
The findings showed that the enzymatic method had good linearity, excellent precision, and a high correlation with the higher-order comparator method, the HS/GC method, in the ethanol analysis.
Subject
Biochemistry (medical),Clinical Biochemistry,Molecular Biology,Biochemistry
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