Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells-Reference-Cited by-同舟云学术

Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

Published:2021-01-01 Issue:1 Volume:12 Page:132-143
ISSN:1868-503X
Container-title:Biomolecular Concepts
language:en
Short-container-title:

Author:

Potter Matthew L.¹,Smith Kathryn²,Vyavahare Sagar²,Kumar Sandeep²,Periyasamy-Thandavan Sudharsan³,Hamrick Mark¹²⁴,Isales Carlos M.⁴⁵,Hill William D.⁶⁷,Fulzele Sadanand¹²⁴⁵¹

Affiliation:

1. Department of Orthopedics , Augusta University , Augusta , GA

2. Department of Cell Biology and Anatomy , Augusta University , Augusta , GA

3. Georgia Cancer Center , Augusta University , Augusta , GA , United States

4. Institute of Healthy Aging , Augusta University , Augusta , GA

5. Departments of Medicine , Augusta University , Augusta , GA

6. Department of Pathology and Laboratory Medicine , Medical University of South Carolina , Charleston , SC

7. Ralph H Johnson Veterans Affairs Medical Center , Charleston , SC ,

Abstract

Abstract Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

Publisher

Walter de Gruyter GmbH

Subject

Cellular and Molecular Neuroscience,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://www.degruyter.com/document/doi/10.1515/bmc-2021-0015/pdf

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