Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay-Reference-Cited by-同舟云学术

Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay

Published:1999-01-01 Issue:7-8 Volume:380 Page:
ISSN:1431-6730
Container-title:Biological Chemistry
language:
Short-container-title:

Author:

Henke E.,Bornscheuer U. T.

Publisher

Walter de Gruyter GmbH

Subject

Clinical Biochemistry,Molecular Biology,Biochemistry

Link

https://www.degruyter.com/document/doi/10.1515/BC.1999.128/pdf

Reference21 articles.

1. Purification and Properties of a Thermostable Esterase ofBacillus stearothermophilusProduced by RecombinantBacillus brevis

2. Design by Directed Evolution

3. Directed Evolution of Enzymes

4. Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones

5. Quantitative analyses of biochemical kinetic resolutions of enantiomers

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