MicroRNA-29a and microRNA-122 expressions and other inflammatory markers among obese children with diabetes
Author:
Bayoumy Nervana M.K.1, El-Shabrawi Mohamed M.2, Elsayed Wafaa3, Kamal Hagar A.2, abdelmaogood Asmaa K.2, Ahmed-Maher Shymaa4ORCID, Omar Hamdy H.5ORCID, Abdel-Rahman Ahmed3
Affiliation:
1. Physiology Department, College of Medicine, Center of Excellence in Thrombosis & Hemostasis , King Saud University , Riyadh , Kingdom of Saudi Arabia 2. Clinical and Chemical Pathology Department, Faculty of Medicine , Suez Canal University , Ismailia , Egypt 3. Pediatric Department, Faculty of Medicine , Suez Canal University , Ismailia , Egypt 4. Medical Biochemistry and Molecular Biology Department, Faculty of Medicine , Suez Canal University , Ismailia , Egypt 5. Internal Medicine Department, Faculty of Medicine , Suez Canal University , Ismailia , Egypt
Abstract
Abstract
Objectives
This study was conducted to study the expression of both microRNA-29a and microRNA-122, and serum levels of sestrin-2, interleukin-6 (IL-6), and other inflammatory markers among obese children with/and without diabetes mellitus.
Methods
One hundred obese children with diabetes in addition to 100 age- and sex-matched obese children without diabetes, and 100 age- and sex-matched apparently healthy children were included in the study. Expressions of both microRNA-29a and microRNA-122, and serum levels of sestrin-2, IL-6, tumor necrosis factor-α (TNF-α), and high sensitive-CRP (hsCRP) were measured for all included study populations.
Results
Study results showed that the expressions of both microRNA-29a and microRNA-122, serum levels of IL-6, TNF-α, and hsCRP were significantly higher among obese children with diabetes in comparison to both obese children without diabetes and healthy children. In contrast, serum sestrin level was significantly low among obese children with diabetes in comparison to the other study populations. Expressions of both microRNA-29a and microRNA-122 were correlated with waist circumference, BMI, total cholesterol, triglycerides, LDL-cholesterol, HbA1c, c-peptide, glucose, insulin, homeostatic model assessment-insulin resistance (HOMA-IR), IL-6, hsCRP, and TNF-α among obese children with diabetes. However, serum sestrin-2 level was correlated inversely with these parameters. Higher expressions of both microRNA-29a and microRNA-122 among obese children either with or without diabetes mellitus (DM) can suggest their roles in the development of obesity among children.
Conclusions
The study results can hypothesize that down-regulation of these micro-RNAs may solve this health problem with its sequelae, a hypothesis that needs more studies.
Publisher
Walter de Gruyter GmbH
Subject
Endocrinology,Endocrinology, Diabetes and Metabolism,Pediatrics, Perinatology and Child Health
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