DNA Profiling, Pedigree Lineage Analysis and Monitoring in the Australian Breeding Program of Radiata Pine

Author:

Bell J. C.1,Powell M.2,Devey M. E.1,Moran G. F.1

Affiliation:

1. CSIRO Forestry and Forest Products. PO Box E4008 Kingston ACT 2604, Canberra , Australia

2. Southern Tree Breeding Association, PO Box 1811 Mount Gambier SA 5290, Australia

Abstract

Abstract Ten microsatellite loci were used to DNA profile 215 first and second generation breeding clones in the Australian radiata pine breeding program. The clones were essentially unique from one another and these DNA profiles were used to examine the sexual and asexual phases in the breeding program. Only 2.6% of ramets from 25 breeding clones were incorrect whereas 8.4% of progeny from 10 families were genetically incorrect. The lineages of second generation clones were checked for DNA profiles that were available for putative parents. Some parents appear to be incorrect on the basis of the DNA profiles and further sampling will be required to confirm these results across the breeding program. Microsatellite markers can now enable most questions to be resolved in breeding programs and also will allow novel breeding strategies to be used in the future.

Publisher

Walter de Gruyter GmbH

Subject

Genetics,Forestry

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