Determination of petroleum biodegradation by bacteria isolated from drilling fluid, waste mud pit and crude oil

Abstract

AbstractObjective:In this study, the aim was to isolate and identify bacterial strains in crude oil, drilling fluid and waste mud pit samples collected from the same oil field, determine the petroleum biodegradation and find the most effective bacteria in the samples in petroleum biodegradation.Methods:The contents of crude oil, drilling fluid and waste mud pit samples were enriched in appropriate conditions. Upon identification of the isolated bacteria, the incubations in petroleum containing media were performed at 150 rpm at 30°C for 7 days. Petroleum biodegradations by bacteria were measured by using colorimetric, spectrophotometric and gravimetric methods.Results:were isolated from the crude oil sample;Conclusion:In literature, no study was encountered showing biodegradation efficiencies of