FAST-EM array tomography: a workflow for multibeam volume electron microscopy-Reference-Cited by-同舟云学术

FAST-EM array tomography: a workflow for multibeam volume electron microscopy

Published:2024-04-01 Issue:1 Volume:1 Page:49-64
ISSN:2942-3899
Container-title:Methods in Microscopy
language:en
Short-container-title:

Author:

Kievits Arent J.¹^ORCID,Duinkerken B. H. Peter²^ORCID,Lane Ryan¹^ORCID,de Heus Cecilia³^ORCID,van Beijeren Bergen en Henegouwen Daan²,Höppener Tibbe¹,Wolters Anouk H. G.²,Liv Nalan³^ORCID,Giepmans Ben N. G.²^ORCID,Hoogenboom Jacob P.¹^ORCID

Affiliation:

1. Department of Imaging Physics , Delft University of Technology , Delft , The Netherlands

2. Department of Biomedical Sciences , University Medical Center Groningen , Groningen , The Netherlands

3. Center for Molecular Medicine , University Medical Center Utrecht , Utrecht , The Netherlands

Abstract

Abstract Elucidating the 3D nanoscale structure of tissues and cells is essential for understanding the complexity of biological processes. Electron microscopy (EM) offers the resolution needed for reliable interpretation, but the limited throughput of electron microscopes has hindered its ability to effectively image large volumes. We report a workflow for volume EM with FAST-EM, a novel multibeam scanning transmission electron microscope that speeds up acquisition by scanning the sample in parallel with 64 electron beams. FAST-EM makes use of optical detection to separate the signals of the individual beams. The acquisition and 3D reconstruction of ultrastructural data from multiple biological samples is demonstrated. The results show that the workflow is capable of producing large reconstructed volumes with high resolution and contrast to address biological research questions within feasible acquisition time frames.

Funder

EU-REACT

ZonMw

Exacte en Natuurwetenschappen

NXTGEN HIGHTECH

Publisher

Walter de Gruyter GmbH

Link

https://www.degruyter.com/document/doi/10.1515/mim-2024-0005/pdf

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