An Alternative to the Mixed Probe Method in DNA Hybridization: Synthetic "lure" Nucleotide for the Ambiguous Position of Codons

Abstract

Abstract Two deoxynucleoside analogues, 1-(2-deoxy-β-D-ribofuranosyl)-2-oxo-4-imidazoline-4-carbox-amide (1) and 5-carbamoyl-2′-deoxyuridine (2), were synthesized and incorporated into decamer deoxynucleotides, which were hybridized to their complementary strands. The duplices in which 1 paired with A or G showed higher melting temperatures (Tm's) than those containing G -T or A -C mismatch, although lower than the Tm of intact totally complementary duplex. The incor­poration of 2 into a strand resulted in extreme destabilization of the duplex.