Effects of chromium picolinate on the parameters of oxidative and chromosomal DNA damage in rabbits

Abstract

Abstract Objectives: This study investigated the effects of chromium chloride (CrCl3·6H2O), chromium picolinate (CrPic) and picolinic acid on malondialdehyde (MDA), 8-hidroksi-2′-deoksiguanosine (8-OHdG) and genome damage in rabbits. Material and methods: Forty, New Zealand rabbits were equally assigned to four groups which received either distilled water or 20 mg/day Cr (CrC13·6H20), 200 μg/day CrPic and 1400 μg/day picolinic acid for 50 days. On the 25th and 50th days, MDA and 8-OHdG levels and the apoptotic-necrotic cells, micronucleus (MN), micronuclear buds (NBUD) and nucleoplasmic bridges (NPB) frequencies and on the 50th day, serum Cr and liver MDA levels were determined. Results: CrPic increased live weight and feed consumption. On the 25th day of treatment, decreases were detected in MDA levels and MN, NPK and NBUD frequencies in CrPic and picolinic acid groups, and in 8-OHdG levels in CrCl3·6H2O and CrPic groups. Slight or significant differences were determined in all investigated parameters between the measurements of on days 25 and 50. Conclusion: Improvements in of MDA and 8-OHdG levels and genome damage indicators due to CrPic and picolinic acid on the 25th day of the treatment may show that short term of CrPic supplementation reduces oxidative and chromosomal DNA damage in rabbits.