Anti-inflammatory effect of D-pinitol isolated from the leaves of Colutea cilicica Boiss et Bal. on K562 cells

Abstract

AbstractAim:D-pinitol, a natural compound has shown various biological and pharmacological effects. Last studies are focused on the determination of its further pharmacological activities including mainly biological activity. Therefore, isolation of D-pinitol from the leaves ofMaterials and methods:Isolation of D-pinitol was performed by column chromatography. Chemical structure of the compound was confirmed by spectroscopic methods includingResults:Stimulation of cells with D-pinitol (0–80 μM) was observed for 24, 48 and 72 h. It is determined that D-pinitol inhibited protein expression of Cox-2 in K562 cells. We observed that Poly (ADP-ribose) polymerase (PARP) protein expression did not change, but Cox-2 protein expression reduced with non-cytotoxic concentrations of D-pinitol.Conclusion:It is concluded that D-pinitol did not affect cell proliferation and apoptosis in K562 cells however reduced the inflammation, significantly. These results show that D-pinitol may be anti-inflammatory agent for the treatment of K562 cells.