Author:
Phadnis Pradeep,Dey Sarkar Purnima,Rajput Mithun Singh
Abstract
Abstract
Background
Initial evidences have shown that diabetes mellitus occurs concomitantly
with obsessive-compulsive disorder (OCD) symptomatology. Serotonergic
psychiatric therapy posits that serotonin is a central character in the
management of OCD. Hence, it is worth investigating novel chemical entities
affecting the serotonergic system for targeting OCD. An isoflavonoid
phytoestrogen, genistein, has been recognized as of great pharmacological value
especially for protecting neurodegeneration, depression (serotonin regulation),
and diabetes. The effectiveness of genistein pretreatment on the symptoms of
OCD in streptozotocin-induced diabetic mice is investigated in this study. We
also evaluate the probable involvement of the serotonergic system.
Methods
Groups of diabetic mice were treated with genistein at the dose of 5.0 and
10.0 mg/kg (intraperitoneal, twice daily, 14 days), and symptoms of OCD were
assessed by the marble-burying behavior, in comparison with the standard drug
fluoxetine. Neurochemical assessment of the serotonergic ratio
5-hydroxyindole-3-methoxyphenylacetic acid/5-hydroxytryptamine (5-HIAA/5-HT) in
the cortical region of the brain was performed using HPLC (high-pressure liquid
chromatography).
Results
Chronic treatment with genistein significantly recovered
[F(6, 35)=53.00, p<0.0001,
R2=0.9008] the symptoms of
OCD as assessed by marble burying behavior in normal and diabetic mice.
Locomotor performance was not influenced by the diabetic condition or any
associated treatment. The turnover of serotonin neurotransmission (5-HIAA/5-HT)
was significantly boosted in the diabetic condition; genistein treatment
dragged it [F(6, 35)=35.75, p<0.0001,
R2=0.8597] toward the
respective control.
Conclusions
Genistein supplementation might be a potential therapeutic line for the
management and/or prevention of diabetes-associated OCD
symptomatology.
Subject
Drug Discovery,Pharmacology,General Medicine,Physiology
Cited by
12 articles.
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