Affiliation:
1. Department of Physiology , College of Medicine of the University of Lagos , Lagos , Nigeria
2. Department of Pharmacology, Therapeutics and Toxicology , College of Medicine, University of Lagos , Lagos , Nigeria
Abstract
Abstract
Objectives
This study aimed to access the protective effects of d-ribose-l-cysteine (DRLC) on cyclophosphamide (CPA) induced gonadal toxicity in male rats.
Methods
Forty-eight male Sprague-Dawley rats were divided into six groups of eight rats each. Group I the control, received distilled water (10 ml/kg), Group II received a single dose of CPA 100 mg/kg body weight intraperitoneally (i.p), Groups III and IV received a single dose of CPA at 100 mg/kg (i.p) and then were treated with DRLC at 200 mg/kg bodyweight (b.w) and 400 mg/kg b.w for 10 days, respectively. Rats in Groups V and VI received DRLC at 200 and 400 mg/kg b.w for 10 days, respectively. DRLC was administered orally.
Results
Results showed that CPA increased percentage of abnormal sperm cells and reduced body weight, sperm count, sperm motility, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and testosterone levels (p<0.05). CPA also induced oxidative stress as indicated by the increased malondialdehyde (MDA) content and reduced activities of the oxidative enzymes measured (p<0.05). Liver enzymes were elevated while the blood cells production was decreased in the rats administered CPA. DRLC supplementation enhanced the antioxidant defence system as indicated in the reduced MDA levels and increased activities of the antioxidant enzymes when compared with CPA (p<0.05). Bodyweight, sperm count, sperm motility, FSH, and testosterone levels were increased in the CPA + DRLC II group compared with CPA (p<0.05).
Conclusions
The results of this present study showed that DRLC has a potential protective effect on CPA-induced gonadotoxicity.
Subject
Drug Discovery,Pharmacology,General Medicine,Physiology
Reference48 articles.
1. Venkatesan, N, Chandrakasan, G. In vivo administration of taurine and niacin modulate cyclophosphamide-induced lung injury. Eur J Pharmacol Environ Toxicol Pharmacol. 1994;292:75–80. https://doi.org/10.1016/0926-6917(94)90028-0.
2. Kanno, TYN, Sensiate, LA, Paula, NAD, Salles, MJS. Toxic effects of different doses of cyclophosphamide on the reproductive parameters of male mice. Braz J Pharmaceut Sci. 2009;45:313–9. https://doi.org/10.1590/s1984-82502009000200017.
3. Hall, AG, Tilby, MJ. Mechanisms of action of, and modes of resistance to, alkylating agents used in the treatment of haematological malignancies. Blood Rev. 1992;6:163–73. https://doi.org/10.1016/0268-960x(92)90028-o.
4. Allison, AC. Immunosuppressive drugs: the first 50 years and a glance forward. Immunopharmacology. 2000; 47:63–83. https://doi.org/10.1016/s0162-3109(00)00186-7.
5. Medubi, L, Ama, C, Medubi, O, Onwosu, NC, Lawal, O, Osinubi, A. D-Ribose-L-cysteine-rich supplement attenuates doxorubicin-induced impaired spermatogenesis, testicular steroidogenesis and redox status in Sprague-Dawley rats. Afr J Biomed Res. 2019;22:179–85.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献