Meta-lens light-sheet fluorescence microscopy for <i>in vivo</i> imaging-Reference-Cited by-同舟云学术

Meta-lens light-sheet fluorescence microscopy for in vivo imaging

Published:2022-02-21 Issue:9 Volume:11 Page:1949-1959
ISSN:2192-8614
Container-title:Nanophotonics
language:en
Short-container-title:

Author:

Luo Yuan¹,Tseng Ming Lun²,Vyas Sunil¹,Hsieh Ting-Yu¹,Wu Jui-Ching³,Chen Shang-Yang¹,Peng Hsiao-Fang¹,Su Vin-Cent⁴,Huang Tzu-Ting⁵,Kuo Hsin Yu¹,Chu Cheng Hung¹,Chen Mu Ku⁶^ORCID,Chen Jia-Wern¹,Chen Yu-Chun¹,Huang Kuang-Yuh⁵,Kuan Chieh-Hsiung¹,Shi Xu⁷,Misawa Hiroaki⁷,Tsai Din Ping⁸^ORCID

Affiliation:

1. National Taiwan University, Institute of Medical Device and Imaging , No. 1 Ren Ai Rd. Sect. 1 , Taipei , 10051 , Taiwan, ROC

2. Institute of Electronics, National Yang Ming Chiao Tung University , Hsinchu , Taiwan, ROC

3. Department of Clinical Laboratory Sciences and Medical Biotechnology , National Taiwan University , No. 1., Chang-Te St. , Taipei , 100 , Taiwan, ROC

4. Electrical Engineering , National United University , No. 2, Lienda , Miaoli , 36003 , Taiwan, ROC

5. Mechanical Engineering , National Taiwan University , No. 1, Sec. 4, Roosevelt Rd. , Taipei , 10617 , Taiwan, ROC

6. Department of Electrical Engineering , City University of Hong Kong , Kowloon , Hong Kong

7. Hokkaido University , Sapporo , Hokkaido , Japan

8. Department of Electrical Engineering , The Hong Kong Polytechnic University, City University of Hong Kong , Kowloon , Hong Kong

Abstract

Abstract Light-sheet fluorescent microscopy has become the leading technique for in vivo imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode Caenorhabditis elegans (C. elegans). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live C. elegans with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire in vivo images of biological specimens with high resolution.

Publisher

Walter de Gruyter GmbH

Subject

Electrical and Electronic Engineering,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biotechnology

Link

https://www.degruyter.com/document/doi/10.1515/nanoph-2021-0748/pdf

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