Fluorescence-based thermometry for precise estimation of nanoparticle laser-induced heating in cancerous cells at nanoscale

Author:

Peltek Oleksii O.1,Ageev Eduard I.1ORCID,Talianov Pavel M.1,Mikushina Anna D.2,Epifanovskaya Olga S.3,Dubavik Aliaksei4,Veiko Vadim P.1,Lepik Kirill3,Zuev Dmitry A.1,Timin Alexander S.1,Zyuzin Mikhail V.1ORCID

Affiliation:

1. School of Physics and Engineering , ITMO University , Lomonosova 9, 191002 , St. Petersburg , Russian Federation

2. Laboratory of Renewable Energy Sources , Alferov University , Khlopina 8/3, 194021 , St. Petersburg , Russian Federation

3. RM Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantation, Pavlov University , Lva Tolstogo 6/8, 191144 , St. Petersburg , Russian Federation

4. Faculty of Photonics, Center of Optical Information Technologies , ITMO University , Birzhevaya liniya 4, 199034 , St. Petersburg , Russian Federation

Abstract

Abstract Photothermal therapy (PTT) has attracted increasing interest as a complementary method to be used alongside conventional therapies. Despite a great number of studies in this field, only a few have explored how temperatures affect the outcome of the PTT at nanoscale. In this work, we study the necrosis/apoptosis process of cancerous cells that occurs during PTT, using a combination of local laser heating and nanoscale fluorescence thermometry techniques. The temperature distribution within a whole cell was evaluated using fluorescence lifetime imaging microscopy during laser-induced hyperthermia. For this, gold nanorods were utilized as nanoheaters. The local near-infrared laser illumination produces a temperature gradient across the cells, which is precisely measured by nanoscale thermometry. This allows one to optimize the PTT conditions by varying concentration of gold nanorods associated with cells and laser power density. During the PTT procedure, such an approach enables an accurate determination of the percentages of apoptotic and necrotic cells using 2D and 3D models. According to the performed cell experiments, the influence of temperature increase during the PTT on cell death mechanisms has been verified and determined. Our investigations can improve the understanding of the PTT mechanisms and increase its therapeutic efficiency while avoiding any side effects.

Funder

Russian Science Foundation

Publisher

Walter de Gruyter GmbH

Subject

Electrical and Electronic Engineering,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biotechnology

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