Genetic analysis of Ulva (Ulvaceae, Chlorophyta) type specimens resolves northeast Pacific blade-forming species
Author:
Hughey Jeffery R.1ORCID, Miller Kathy Ann2ORCID, Gabrielson Paul W.3ORCID
Affiliation:
1. Division of Mathematics, Science, and Engineering , Hartnell College , Salinas , CA 93901 , USA 2. University Herbarium, University of California at Berkeley , 1001 Valley Life Sciences Building #2465, 94720-2465 , Berkeley , CA , USA 3. Biology Department and Herbarium , University of North Carolina at Chapel Hill , Coker Hall CB 3280, 27599-3280 , Chapel Hill , NC , USA
Abstract
Abstract
Misapplication of Ulva epithets in GenBank has led to confusion in the scientific literature and community. To solve some of the problems, targeted DNA sequencing of plastid encoded rbcL gene amplicons or high-throughput sequencing was performed on all blade-forming Ulva type specimens from the northeast Pacific. Recently collected specimens from at or near type localities were also analyzed for some taxa. Based on these genetic analyses, we confirmed currently recognized species: U. californica, with U. angusta and U. scagelii as heterotypic synonyms, U. stenophylla, U. taeniata, and U. tanneri. Ulva dactylifera, currently considered a synonym of U. taeniata based on morpho-anatomy, is recognized as a distinct species, as is U. expansa whose type specimen was sequenced in 2018. All but two of the ITS, rbcL and tufA sequences in GenBank that were labeled U. californica were correctly named, in contrast to U. taeniata, for which only one of 14 sequences was correctly labeled. These results show that DNA sequencing of Ulva type specimens is essential for the correct application of names.
Publisher
Walter de Gruyter GmbH
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