Separation of 90Nb from zirconium target for application in immuno-PET

Author:

Radchenko V.1,Filosofov D. V.2,Bochko O. K.2,Lebedev N. A.2,Rakhimov A. V.2,Hauser H.3,Eisenhut M.3,Aksenov N. V.4,Bozhikov G. A.4,Ponsard B.5,Roesch F.1

Affiliation:

1. Institute of Nuclear Chemistry, Johannes Gutenberg-University Mainz, Fritz-Strassmann-Weg 2, D-55128 Mainz, Germany

2. Dzhelepov Laboratory of Nuclear Problems, Joint Institute of Nuclear Research, Joliot-Curie 6, 141980, Dubna, Moscow region, Russian Federation

3. Radiopharmaceutical Chemistry, German Cancer Research Center, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany

4. Flerov Laboratory of Nuclear Reactions, Joint Institute of Nuclear Research, Joliot-Curie 6, 141980, Dubna, Moscow region, Russian Federation

5. Institute of Nuclear Materials Science, BR2 Reactor, Radioisotopes and NTD Silicon Production, Belgian Nuclear Research Centre, SCKCEN, Boeretang 200, BE-2400 Mol, Belgium

Abstract

Abstract Fast progressing immuno-PET asks to explore new radionuclides. One of the promising candidates is 90Nb. It has a half-life of 14.6 h that allows visualizing and quantifying biological processes with medium and slow kinetics, such as tumor accumulation of antibodies and antibodies fragments or drug delivery systems and nanoparticles. 90Nb exhibits a positron branching of 53% and an average kinetic energy of emitted positrons of E mean =0.35 MeV. Currently, radionuclide production routes and Nb V labeling techniques are explored to turn this radionuclide into a useful imaging probe. However, efficient separation of 90Nb from irradiated targets remains in challenge. Ion exchange based separation of 90Nb from zirconium targets was investigated in systems AG 1 × 8 – HCl/H2O2 and UTEVA-HCl. 95Nb (t 1/2 = 35.0 d), 95Zr (t 1/2 = 64.0 d) and 92m Nb (t 1/2 = 10.15 d) were chosen for studies on distribution coefficients. Separation after AG 1 × 8 anion exchange yields 99% of 90/95Nb. Subsequent use of a solid-phase extraction step on UTEVA resin further decontaminates 90/95Nb from traces of zirconium with yields 95% of 90/95Nb. A semi-automated separation takes one hour to obtain an overall recovery of 90/95Nb of 90%. The amount of Zr was reduced by factor of 108. The selected separation provides rapid preparation (< 1 h) of high purity 90Nb appropriate for the synthesis of 90Nb-radiopharmaceuticals, relevant for purposes of immuno-PET. Applying the radioniobium obtained, 90/95Nb-labeling of a monoclonal antibody (rituximab) modified with desferrioxamine achieved labeling yields of >90% after 1 h incubation at room temperature.

Publisher

Walter de Gruyter GmbH

Subject

Physical and Theoretical Chemistry

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