Author:
Macholán Lumír,Jílková Dagmar
Abstract
A sensor method has been worked out for the determination of spermine and spermidine in concentrations 0.02-0.5 mmol l-1. The method is based on amperometric measurement of the consumption of oxygen and the formation of hydrogen peroxide in a reaction catalyzed by crosslinked polyamine oxidase from maize. One determination takes 1 to 2 min, with phosphate at least 8 months. The optimum conditions for the analysis are described and the applicability of the sensor to urine samples is dealt with.
Publisher
Institute of Organic Chemistry & Biochemistry
Cited by
15 articles.
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