Changes in ribosomes from ischemic spinal cord

Abstract

The paper deals with changes in polyribosomal profiles and with incorporation of amino acids into spinal cord ribosomes of dogs after ligature of their lumbar aortas. The polyribosomal profile was unchanged after 80 minutes' ischemia, but ischemia for two 40 min periods with 40 min recirculation in between resulted in disintegration of polysomes into their ribosomal subunits. Recirculation for 48 h was sufficient for return to control values. The incorporation activity of ribosomes in vitro was unchanged, but cell saps isolated from ischemic cells reduced incorporation of amino acids into ribosomes. The results are discussed in terms of concentration changes of adenine nucleotides under the given experimental conditions.