Synthesis of the uracil analog of thuringiensin and its inhibitory effect on DNA-dependent RNA polymerase of Escherichia coli

Abstract

An analog of thuringiensin (II), containing uracil instead of adenine, was synthetized by the method employed for thuringiensin. Similarly to the parent substance this analog inhibits the DNA-dependent RNA polymerase of Escherichia coli, simultaneously competing with the natural substrate, i.e. UTP. The inhibition constant (Ki = 30 μm) shows that the uracil analog of thuringiensin is an inhibitor of RNA polymerase roughly as efficient as thuringiensin.