Preparation of α-5-Aza-2'-deoxy-[6-3H]cytidine

Abstract

α-5-Aza-2'-deoxy cytidine was labeled by tritium on the C-6 of the heterocyclic triazine ring. The structure of the α-5-aza-2'-deoxy-[6-3H]cytidine and the position of the label was proved by 3H and 1H NMR. The specific activity was 0.71 TBq mmol-1 (19.2 Ci mmol-1) and radiochemical purity was >99%. The long term stability of the product during the storage at -21 and -72 °C was followed by radio-HPLC.