Polarographic and voltammetric determination of genotoxic 2-aminofluoren-9-one at mercury electrodes

Abstract

Electrochemical behavior of genotoxic 2-aminofluoren-9-one (2-AFN) was investigated by DC tast polarography (DCTP) and differential pulse polarography (DPP), both at a classical dropping mercury electrode (DME), and by DC voltammetry (DCV), differential pulse voltammetry (DPV), and adsorptive stripping differential pulse voltammetry (AdSDPV), all at a miniaturized hanging mercury drop minielectrode (HMDmE), in buffered aqueous-methanolic solutions. Optimum conditions were found for the determination of 2-AFN by DCTP at DME in the concentration range from 1 × 10–6to 1 × 10–4mol l–1(with a limit of quantification (LQ) of 5 × 10–7mol l–1), by DPP at DME (from 1 × 10–7to 1 × 10–4mol l–1;LQ ≈ 1 × 10–7mol l–1), by DCV and DPV at HMDmE (both from 1 × 10–7to 1 × 10–4mol l–1;LQs ≈ 2 × 10–7and 1 × 10–7mol l–1for DCV and DPV, respectively), and by AdSDPV at HMDmE (from 2 × 10–9to 1 × 10–7mol l–1;LQ ≈ 4 × 10–9mol l–1). Practical applicability of the developed methods was verified on the direct determination of 2-AFN in model samples of drinking and river water in nanomolar to micromolar concentrations.