Inhibition of Biosynthesis and Biochemical Modulation of N-Acylneuraminic Acid (Biochemical Engineering of Sialoconjugates). A Review

Abstract

The key enzyme of sialic acid biosynthesis is the bifunctional UDP-GlcNAc 2-epimerase/ ManNAc kinase. Novel inhibitors of this enzyme have been synthesized. TheN-acyl side chain of sialic acid can be biochemically engineered by incubating cells with non-naturalN-acylmannosamine analogues such asN-propionylmannosamine and related compounds. These modified sialic acids lead to various biological changes, such as stimulation of T-lymphocyte proliferation, inhibition of the uptake of influenza A virus, stimulation of neuritic growth, increased expression of sialyl-Lewisxand altered adhesion. A review with 41 references.