Author:
Mislovičová Danica,Gemeiner Peter,Ďurišová Viera
Abstract
The effect of protein admixtures and concentration of immobilized dye on the interaction of lactate dehydrogenase (LDH) with Cibacron Blue-bead cellulose has been studied by means of zonal chromatography with Cibacron Blue-dextran T 10 as the mobile ligand. The influence of both variables on the values of dissociation constants of complexes immobilized dye-LDH (KI-L) and mobile dye-LDH (KM-L) can be summarized as follows: The values of KI-L remained practically constant and were found to be lower than those of KM-L. The differences found in KI-L and KM-L values should help to estimate the contribution of nonspecific interaction of the matrix to the total interaction between dye-ligand and the enzyme.
Publisher
Institute of Organic Chemistry & Biochemistry
Cited by
5 articles.
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