Amperometric Determination of Glucose with a Carbon Paste Biosensor

Abstract

A simple glucose biosensor has been developed by bulk modification of a carbon paste electrode with glucose oxidase as a biocomponent and manganese dioxide as a mediator. The sensor was employed as amperometric detector in a flow-injection system at 21 °C in 0.2 M phosphate buffer (pH 7.5). At an applied working potential of 0.48 V vs Ag/AgCl and a flow rate of 0.2 ml min-1, the sensor exhibited well reproducible amperometric response to glucose. A linear relation between the peak current and the analyte concentration was found between 20 and 500 mg l-1 glucose with a detection limit (3σ) of 10.5 mg l-1 glucose. The sensor can be operated continuously for 12 h without any loss in the signal height and can be used for the determination of glucose in white wine samples.