Evaluation of Flurbiprofen's anticonvulsant properties in pilocarpine-induced convulsions in mice

Abstract

Background: Flurbiprofen is a nonsteroidal anti-inflammatory drug, it has non-selective COX inhibition properties, some NSAIDS has an anticonvulsant effect and benefit as an analgesic in seizure patient. Objective: we aimed to evaluate the possible anticonvulsant effect of flurbiprofen in mice. Methods: We used 25 mature male mice for our study, the mice were divided into five groups, then to prevent peripheral cholinergic activation, mice in all groups were injected intraperitoneally with atropine sulphate (1 mg/kg) subcutaneously one hour after dosing. Seizures were induced ten minutes later with a 300 mg/kg i.p. injection of pilocarpine. After the pilocarpine injection, the animals were monitored for 1 hour. seizures   severity was tested by the Racine scale. Results: flurbiprofen at 0, 10, 20 and 40 mg/kg orally causes a significant decrease in the onset of convulsion and a considerable decrease in the duration of convulsion in comparison with the control group. Flurbiprofen at 20 and 40 mg/kg orally inhibited seizure scores in pilocarpine-treated mice in a dose-dependent manner. High-dose of flurbiprofen (40 mg/kg) significantly reduced seizure scores, similar to diazepam. Conclusion: flurbiprofen has potential anticonvulsant properties in mice models, further study must be conducted to assess the primary mechanism of action.