Potential Lutein Extract of Broccoli (Brassica oleracea L. var. italica) as Antiradical Abts (2,2 - Azinobis Acid, 3-Ethyl Benzothiazoline-6-Sulfonic Acid)

Abstract

<p class="Els-Abstract-text">Broccoli (<em>Brassica oleracea</em> L. var. italica) is one vegetable that is often consumed by people with high nutrient content i.e protein, fat, vitamin C, fiber, potassium and calcium. Carotenoids and glutathione are contained in broccoli that are antioxidants. Lutein is a carotenoid group contained in broccoli is capable of reducing the accumulated products of free radicals in the body. The purpose of this research is to extract and identify compounds lutein from flowers of broccoli and test its potential as antiradical to the compound ABTS (2,2-Azinobis acid, 3-ethyl benzatiazolin-6-Sulfonat). Identification of lutein compounds qualitatively by TLC showed Rf value of 0.72 cm. Analysis of Lutein by HPLC showed a peak at the retention time of 3:03 min, approaching retention time of lutein standard 3:01 min. Test of potency of antiradical ABTS showed that lutein extract from flower of broccoli has antioxidant activity with IC₅₀ value of 157.527 8 µg · mL^–1.  Fractionation of lutein extract using column chromatography with Silica gel 60 as stationary phase and mixture of methanol : chloroform : n-hexane (4:20:8) as mobile phase resulted in 4 fractions with antioxidant activity increased. Fraction 3 has the highest antiradical ABTS activity with IC₅₀ 39.584 2 µg · mL^–1. Vitamin E is used as a standard had antiradical ABTS activity IC₅₀ 5.303 6 µg · mL^–1.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> ABTS ; anti-radical; Broccoli (<em>Brassica oleracea </em>L. var<em>.</em><em> </em>italica); lutein.</p></div>