Affiliation:
1. ge and Hospital, Navi Mumbai, Maharashtra
2. Bharati Vidyapeeth Dental College and Hospital, Navi Mumbai, Maharashtra
Abstract
Introduction
To assess the levels of glutathione peroxidase in the saliva of subjects with generalized stage III periodontitis before and after periodontal treatment.
Material and Methods
This study, compares salivary glutathione peroxidase levels in generalized stage III periodontitis subjects before and after therapy. A total of 20 participants were selected. The clinical and biochemical evaluation was done at specific time intervals as described. The data was collected and analyzed. Levels of dental plaque and gingival index were recorded at baseline and 2 months post periodontal therapy. Salivary glutathione peroxide levels were assessed by ELISA kit at baseline and 2 months post periodontal therapy.
Results
At baseline, the mean plaque index scores were found to be 2.5585 ± 0.06685 (SD). After 2 months, the values were found to have reduced to 0.9080 ± 0.04224. At baseline, the mean gingival index scores were found to be 2.5475 ± 0.3380 (SD). After 2 months, the values were found to have reduced to 0.8700 ± 0.1676. At baseline, the mean glutathione peroxidase levels were found to be 5.1595 ± 0.3713 (SD). After 2 months, the values were found to have reduced by 4.4040 ± 1.1142 (SD).
Conclusions
Glutathione peroxidase level was elevated in disease conditions and is associated with the free radical formation in tissue inflammation. Following periodontal therapy, glutathione peroxidase levels are lowered significantly, indicating reduced tissue inflammation.
Key-words: Antioxidants, Biomarkers, ELISA, Glutathione Peroxidase, Saliva
Publisher
Indian Dental Association
Reference16 articles.
1. Smitha S, Biju T, Rahul B, Nina S. Comparative Evaluation of Salivary and Gingival Tissue Glutathione Peroxidase Levels in Subjects with Healthy Periodontium and Chronic Periodontitis. Sch J Dent Sc. 2017;4(5):211-215. doi:10.36347/sjds.2017.v04i05.001
2. Wei P-F, Ho K-Y, Ho Y-P, Wu Y-M, Yang Y-H, Tsai C-C. The investigation of glutathione peroxidase, lactoferrin, myeloperoxidase and interleukin-1beta in gingival crevicular fluid: implications for oxidative stress in human periodontal diseases. J Periodontal Res. 2004;39(5):287-293. doi:10.1111/j.1600-0765.2004.00744.x
3. Ito Y, Kajkenova O, Feuers RJ, et al. Impaired glutathione peroxidase activity accounts for the age-related accumulation of hydrogen peroxide in activated human neutrophils. J Gerontol A Biol Sci Med Sci. 1998;53(3):M169-75. doi:10.1093/gerona/53a.3.m169
4. Deneke SM, Fanburg BL. Regulation of cellular glutathione. Am J Physiol. 1989;257(4 Pt 1):L163-73. doi:10.1152/ajplung.1989.257.4.L163
5. Huang P, Su T, Wang H. [The relationship between GPx activity in gingival fluid and clinical parameters of adult periodontitis]. Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China J Stomatol. 2000;18(2):106-108.