A Flow Cytometry-Based Method to Determine the Titer of Adenoviruses Expressing an Extraneous Gene
Author:
Affiliation:
1. Oncolys BioPharma, Inc.
2. Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University
Publisher
Pharmaceutical Society of Japan
Subject
Pharmaceutical Science,Pharmacology,General Medicine
Link
https://www.jstage.jst.go.jp/article/bpb/41/10/41_b18-00316/_pdf
Reference28 articles.
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3. 3) Kanegae Y, Makimura M, Saito I. A simple and efficient method for purification of infectious recombinant adenovirus. Jpn. J. Med. Sci. Biol., 47, 157–166 (1994).
4. 4) Mittereder N, March KL, Trapnell BC. Evaluation of the concentration and bioactivity of adenovirus vectors for gene therapy. J. Virol., 70, 7498–7509 (1996).
5. 5) Hitt DC, Booth JL, Dandapani V, Pennington LR, Gimble JM, Metcalf J. A flow cytometric protocol for titering recombinant adenoviral vectors containing the green fluorescent protein. Mol. Biotechnol., 14, 197–203 (2000).
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