Human Hepatocarcinoma Functional Liver Cell-4 Cell Line Exhibits High Expression of Drug-Metabolizing Enzymes in Three-Dimensional Culture

Author:

Kato Ryuji1,Shigemoto Kota1,Akiyama Hiromasa1,Ieda Asaka1,Ijiri Yoshio1,Hayashi Tetsuya1

Affiliation:

1. Laboratory of Cardiovascular Pharmacotherapy and Toxicology, Osaka University of Pharmaceutical Sciences

Publisher

Pharmaceutical Society of Japan

Subject

Pharmaceutical Science,Pharmacology,General Medicine

Reference24 articles.

1. 1) Rodríguez-Antona C, Donato MT, Boobis A, Edwards RJ, Watts PS, Castell JV, Gómez-Lechón MJ. Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica, 32, 505–520 (2002).

2. 2) Donato MT, Lahoz A, Castell JV, Gómez-Lechón MJ. Cell lines: a tool for in vitro drug metabolism studies. Curr. Drug Metab., 9, 1–11 (2008).

3. 3) Hasumura S, Sujino H, Nagamori S, Kameda H. Establishment and characterization of a human hepatocellular carcinoma cell line JHH-4). Hum. Cell, 1, 98–100 (1988).

4. 4) Hamilton GA, Jolley SL, Gilbert D, Coon DJ, Barros S, LeCluyse EL. Regulation of cell morphology and cytochrome P450 expression in human hepatocytes by extracellular matrix and cell–cell interactions. Cell Tissue Res., 306, 85–99 (2001).

5. 5) Oda H, Yoshida Y, Kawamura A, Kakinuma A. Cell shape, cell-cell contact, cell-extracellular matrix contact and cell polarity are all required for the maximum induction of CYP2B1 and CYP2B2 gene expression by phenobarbital in adult rat cultured hepatocytes. Biochem. Pharmacol., 75, 1209–1217 (2008).

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