CULTURE USE PROBLEMS IN SELECTION OF ISOLATED MICROSPORES IN GRAIN

Abstract

Production of haploid plants by culture of isolated microspores is a quick way of obtaining homozygous crop lines. Recessive features of mutant homozygous plants are also possible to determine by this biotechnology. Contrary from anthers culture, in which the presence of anther walls can lead to the development of diploid somatic calli and plants, the microspore culture produces only haploid or dihaploid lines. Isolated microspores culture in addition represents and has a unique identification system for studying the mechanisms of embryogenesis in in vitro culture. The usage of haploid technology extends the genetic basis of wheat breeding, since it allows increasing the frequency of new gene combinations. This technology significantly increases the efficiency of breeding new highly productive varieties of crops. On this basis, it becomes possible to quickly assess the prospects of dihaploids, which significantly improves the efficiency of the selection process. DH plants are completely fertile and, if necessary, may be used as parents or processed as a cultivar. DHs have been widely used for cultivar development, genetic mapping, mutagenesis, and the study of gene functions.