Evidence for a Novel Insecticidally Active Exotoxin Produced By the HD 116 Strain of Bacillus thuringiensis var. morrisoni
Author:
Argauer Robert J.1,
Cantwell George E.1,
Cantelo William W.1
Affiliation:
1. Environmental Chemistry Laboratory, Natural Resources Institute, USDA-ARS, Beltsville, MD 20705
Abstract
Liquid chromatographic and bioassay data provide evidence for a novel exotoxin produced by the HD 116 strain of Bacillus thuringiensis var. morrisoni. The exotoxin from HD 116 differs from the well known beta-exotoxin produced by the HD 59 strain of Bacillus thuringiensis var. thuringiensis. Chromatograms of aliquots of filtered fermentation broth taken before and after inoculation with the organisms HD 116 and HD 59 were compared. Based on retention times of the obtained peaks, chromatographic fractions were obtained and activity against first instar larvae of the Colorado potato beetle (Coleoptera: Chrysomelidae) was determined. Fractions based upon similar retention times that were active from var. morrisoni were not active from var. thuringiensis and vice versa. Our conclusion that the new exotoxin may be a diphosphate analog of thuringiensin is based on a comparison of relative retention times among several adenine nucleotides. We have tentatively named this new extracellular exotoxin sigma-exotoxin.
Publisher
Georgia Entomological Society
Subject
Insect Science,Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献