ISOLATION AND CHARACTERIZATION OF 13-CYCLOPENTANE TRIDEANOIC ACID FROM CYANTHILLIUM CINERIUM (L.) H. ROB. AND IT’S IN–SILICO BASED ANTI-DIABETIC EFFECT

Abstract

Naturally available medicinal plants are found to possess enormous biological properties based on their bioactive constituents present in that plant. Cyanthillium cinereum (L.) H. Rob belongs to Asteraceae family has been reported as anti-microbial, antioxidant, anti- inflammatory properties. This study was aimed to explore the isolation and characterization of bioactive components present in the extracts of Cyanthillium cinereum and its anti-diabetic activities based on in-silico approaches. The methanolic extract of Cyanthillium cinereum was further proceeded to gel chromatography to isolate the active compound and its structure of obtained compound was elucidated by UV-VIS spectrometry, FT-IR spectrum, LC-MS, GC-MS and H1 & C13 NMR studies were carried. Based on these studies, the isolated compound was identified as “13- Cyclopentane Trideanoic acid” for the first time from this plant. The molecular formula for 13- Cyclopentane Trideanoic acid is C18H34O2 and molecular weight is 282.46 g/mol. Further, the ADMET and PASS analysis was carried out for 13- Cyclopentane Trideanoic acid, the results further reveals that this compound showed promising Drug likeliness properties and anti-diabetic activity scores. Thereby, based on these in-silico analysis, we intent to dock this compound with GLUT1, the binding affinity -165.71 showed that 13- Cyclopentane Trideanoic acid was interacting with GLUT1 at their active sites. Taken together, the isolated compound 13-Cyclopentane Trideanoic acid from Cyanthillium cinereum possess antidiabetic effects. In future, the anti-diabetic property of this compound may be explored in other models.