Effect Of Vaccinium Macrocarpon On Candida Albicans Adhesion To Denture Base Resin

Abstract

Objective This study aimed to assess the effectivenessof Vaccinium macrocarpon (cranberry)extract on Candida albicans adhesion tocranberry added denture base biomaterial. Method Different proportions of cranberryextract were incorporated into polymer of polymethyl methacrylate (PMMA) self - cure resin material forfabricationfifty of 4 mm x 4mm x 2mm samples. Group I contained 0 wt/wt% cranberry was used as control group. 0.5wt/wt%, 1.0 wt/wt%, 1.5 wt/wt% and 2 wt/wt% was graded as Group II to V in order.Addition of 0.1mL of Candida albicans suspension was done in each well of tissue culture plate and incubated for 48 hours. 2% cranberrywasused as disinfectant solution in four serial dilutions at undiluted, 1:10, 1:100, 1:1000 ratio. Immersion of resin samples in disinfectant solution was done for 10 minutes. A colony forming unit was measuredafter 0.1mL of the solution was plated on as sabouraud dextrose agar (SDA) plate and cultured for 72 hours. Data were subjected to statistical analysis. Results The mean colony forming units (CFU) per mL showed that there were severalconfluent colonieswith all proportions of cranberry extract added poly methyl methacrylate (CA-PMMA) resin samplesin undiluted and 1:10 dilution state with both distilled water and cranberry disinfectants. While immersion indistilled waterat 1 :100 dilution, mean CFU/mL was 2.99 0.29in all groups of CA-PMMA. In 1 :1000 dilution state, the mean  SD was 117  0.42.Immersion in cranberry solution at 1:100 and 1:1000 showed 2.65 0.66 and 1.26 0.59 for all groups of CA-PMMA. On comparison between distilled water and cranberry as disinfectants, a statistically significant difference was noted with all groups as p < 0.043. Insignificant variation was observed within the groups with both disinfectants as p > 0.05. Conclusion Adherence of Candida albicans to cranberry added self-cure poly methyl methacrylate biomaterial was evidenced to be less irrespective of extract proportions of 1%, 1.5% and 2% in PMMA.Cranberry’s effectiveness as disinfectant showed lesscandida growth when compared to distilled water immersion. This infers that cranberry has anti-adherence property when used both as a polymer constituent material as well as disinfectant solution.